実験的根尖部病巣の発症に関する研究

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Numerous literatures are available on the pathohistology of periapical focus of infection which are formed naturally in the teeth of animals by introduction of pathogenic bacteria. Imai has succeeded to inoculate certain strain of pathogenic bacteria into dog's teeth to establish a periapical focus of infection and has reported patho-histological and microbiological findings obtained. These studies, however, failed to explain the possible mechanism by which the focus of infection was established there. The present study was undertaken to furnish some of more detailed informations about this problem by inoculating cells of a pathogenic strain of staphylococcus that were freshly isolated from the putrescent lesions of the mouth into the lower 1st molars of a total of 26 dogs as experimental material. In the first series of experiment, two groups of the teeth were respectively inoculated with the same amount of the staphylococcal cells twice a week and the first group of teeth was left to keep contact with the antagonizing cusp, while the second group was left apart from coming into normal occlusion after their cusps were opened for the inoculation. Radiographic observation showed that the enlargement of the periodontal membrane space occurred in 83.3% of teeth on the 4th day and in 16.6% on the 7th day respectively after the inoculation in the first group, while in the 2nd group the enlargement of the periodontal membrane occurred on the 4th day in only 22.2% and the majority of the teeth, that is 66.6%, showed the enlargement on the 7th day after the inoculation. In the 2nd series of experiment, comparative study was conducted on the periapical infection of two groups of teeth of which the teeth of one group were infected with the pathogenic cells by way of the exposed pulp and the other group was infected with the cells through direct application into the periapical foramen made artificially after pulp exstirpation. The same amount of the cells was applied twice a week in both cases. The results showed that enlargement of periapical membrane space was observed to occur on the 4th day in 22.2% and on the 7th day in 66.6% in the latter group while in the former group the enargement was not observed on the 4th day, but was observed in 22.2% of teeth on the 7th day after the inoculation. In the 3rd series of experiment, comparison was made between two groups of teeth inoculated with the pathogens by way of the pulp, of which the teeth of one group receiving one inoculation and the teeth of another group receiving inoculation twice a week. The results showed that the enlargement of the periapical membrane space occurred in 33.3% on both the 4th and 7th day for the first group while for the 2nd group the enlargement was not observed at all in the 4th and 7th day but was only observed in 66.6% on the 11th day after the inoculation. Although the time of occurrence of infection did not depend entirely on the amount of cells inoculated into the teeth, all the data showed that the teeth were susceptible to the inoculation without exception and 93.3% had the radiologic sign of periapical infection after 7th day of inoculation. The reduction culture of the cells from the focus of infection and differentiation study of the cell strain by means of the phage typing technique confirmed that the infected focus was establiahed solely by the cell strain originally used. The study was further extended to the examination of periodontal membrane by patho-histological preparations and also by the fluorescein antibody staining method to confirm the exact period at which the focus of infection came to be established. The results showed clear evidence of existing inflammation as well as the presence of cellular products of inoculated staphylococcus in the periodontal tissue by the 6th day of inoculation.
九州歯科学会の論文
1971-03-31

実験的根尖部病巣の発症に関する研究

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