歯周組織における樹状細胞の動態に関する研究
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概要
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The purpose of this paper is to investigate the migration of antigen presenting cells in the periodontal tissue and the submandibular lymph nodes. Lipopolysaccharide (LPS), as an inducer of cell migration, was injected into the gingiva of rats. Thin sections of the periodontal tissue and the submandibular lymph nodes were prepared in the course of time after the injection and stained by an indirect immuno-enzyme method using antibodies ; ED 1 (direct for macrophages), KO 3 (direct for dendritic cells) and OX-3 (direct for Ia antigen). Results are as follows : 1. ED 1 positive cells in the periodontal tissue. ED 1 positive cells widely distributed in the submucosal connective tissue of the buccal side, specially around the blood vessels, and the number was much more than that in the control. Then the cells decreased in number in 3-day-rat, and increased again in 5-day-rat. 2. KO 3-positive cells in the periodontal tissue. KO 3-positive cells widely distributed in the submucosal connective tissue around the alveolar bone in 1-day-rat, and the number was much more than that in the control. The cells also appeared in the epithelium from the granular layer to the basal cells in 3- and 5-day-rat. 3. KO 3-positive cells in submandibular lymph nodes. 1) In the control rats, large KO 3-positive cells moderately distributed in the subcapsular sinus and the paracortex, and a small number of small KO 3-positive cell also distributed in the germinal center. 2) In 1-day-rat, the cells in the subcapsular sinus decreased in number and the cells in the paracortex slightly increased. 3) In 3-day-rat, the cells in the subcapsular sinus slightly increased in number and the cells in the paracortex intensely increased. The cells in the germinal center also increased. 4) In 5-day-rat, the cell distribution was similar with those in 3-day-rat, with a slight decrease in number in the paracortex and with an increase around the germinal center even in the center. 4. Ia antigens in the submandibular lymph nodes. 1) In control rats, a small number of the antigen positive cells distributed in a part of the subcapsular sinus and paracortex. 2) In 1-day-rat, the cells decreased in number in the subcapsular sinus increased in the paracortex. 3) In 3-day-rat, the cells increased in number in the subcapsular sinus and the paracortex. The cells also appeared in the germinal center. 4) In 5-day-rat, the cells decreased in number in the subcapsular sinus, the paracortex and in the germinal center. Thus the results suggest that KO 3-positive cells migrated with antigens from the periodontal tissue to the submandibular lymph nodes through the stream of lymph fluid. The migration of KO 3-positive cells from the subcapsular sinus to the germinal center may show that the cells present the antigens which also transported from the periodontal tissue, to T cells.
- 九州歯科学会の論文
- 1992-06-25