高タンパク食摂取による下顎頭軟骨の成長に関する実験的研究
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概要
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Male Wistar rats of 5 weeks old, corresponding to early childhood, were fed for 3 weeks with standard diet, high protein diet, calcium deficiency diet, and calcium deficiency・high protein diet. Matrix formation of mandibular condyle was investigated. 1. Body weight : First week post-experimentally, significant differences were revealed between control and calcium deficiency groups, high protein and calcium deficiency groups, calcium deficiency and calcium deficiency・high protein groups (p<0.01), high protein and calcium deficiency・high protein groups (p<0.05). At second week, significant differences were revealed between control and high protein groups, high protein and calcium deficiency groups (p<0.01), and between high protein and calcium deficiency・high protein groups, and between calcium deficiency and calcium deficiency・high protein groups (p<0.05). At third week, significant differences were revealed between control and high protein groups, control and calcium deficiency・high protein groups, high protein and calcium deficiency groups, calcium deficiency and calcium deficiency・high protein groups (p<0.01). 2. Radiographic findings : Trabecularity was seen sparsely in high protein group, compared with control group. Calcium deficiency group showed irregular or indistinct trabeculae, when compared with high protein group. Calcium deficiency・high protein group showed the comparable appearance of irregular or indistinct trabeculae with calcium deficiency group. 3. Bone density : Differences were statistically significant between control and calcium deficiency・high protein groups (p<0.05), control and calcium deficiency groups, control and calcium deficiency・high protein groups, high protein and calcium deficiency groups, high protein and calcium deficiency・high protein groups (p<0.01). 4. Histopathological findings : Compared with control group, high protein group showed inhibited chondroclast-mediated resorption and calcification of cartilaginous matrix in hypertrophic chondrocyte layer, especially in erosive area, in high protein group. Compared with high protein group, calcium deficiency group showed expanded hypertropic chondrocytic layer and obviously inhibited erosive area. Calcium deficiency・high protein group showed decreased endochondral ossification resulting from restrained differentiation from chondrocyte to osteocyte. 5. SEM findings : High protein group showed indistinct chondrocytic lacunae and increased resorption, compared with control group. Calcium deficiency group showed many incomplete chondrocytic lacunae, compared with high protein group. Bone formative area was revealed more than resorptive area. Calcium deficiency・high protein group showed indistinct and incomplete chondrocytic lacunae in matrix formation area, compared with calcium deficiency group. From the above results, it was concluded that high protein diet inhibites endochondral ossification and development of condylar cartilage.
- 九州歯科学会の論文
- 1998-08-25