培養癌細胞による抗癌剤の効果を高めるための実験的研究 : KB 細胞に対する Biscoclaurine alkaroid と 5-Fluorouracil, Peplomycin との併用効果

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In the chemotherapy for oral cancers, treatment results have gradually been improving through development of and improvement in drugs and also through studies on amounts and methods of clinical administration. At the same time, these anticancer drugs have various side effects and some drugs can often cause serious disorders of the lung, kidney, heart, myelopathy, and digestive apparatus. In an attempt to minimize the side effects of 5-Fluorouracil (5-FU) and Peplomycin (PEP) frequently used in the chemotherapy for oral cancers and to enhance additive or multiplicative antitumor effects by combining them with non-anticancer drugs, effects of the combination with Cepharanthin (Ceph), a biscoclaurine alkaroid, were investigated in vitro. For the cultivated cancer cells, KB cells of human pharynx cancer origin were used and cultivated for a fixed period. Then, two anticancer drugs, 5-FU and PEP, were combined with Ceph and administered to the cells. The cells were cultivated for 2, 3, and 5 days and their effects on the KB cells were investigated in terms of growth conditions by t-test and morphology. In addition, as to PEP found to be effective through the combination, combination ratios of PEP to Ceph were investigated. The results were as follows : I. Combination effects of various anticancer drugs with Ceph 1. No significant differences in genesistasis (p<0.01) or in cytomorphologic changes were found in the Ceph alone group (group A), as compared with the control group. Between the 5-FU groups (groups B, C, D) and the PEP groups (groups E, F, G), siginificant differences in genesistasis (p<0.01) and in cytomorphologic changes were found. 2. Between the 5-FU alone group (group B) and the 5-FU-Ceph combined groups (groups C, D), no siginificant genesistasis (p<0.01) nor marked cytomorphologic changes were shown. 3. Between the PEP alone group (group E) and the PEP-Ceph combined groups (groups F, G), siginificant differences in genesistasis (p<0.01) and in cytomorphologic changes were shown. 4. The foregoing results indicated that the combined application of PEP and Ceph in the present experimental concentrations enhanced antitumor effect, but the combination of 5-FU with Ceph did not show clear antitumor effect. II. Combination effects of various combination ratios of PEP to Ceph 1. In the combination ratio of 1 : 3 (group H), as compared with the PEP 0.1μg/ml alone group (group E), each of 3-, and 5-day groups showed siginificant genesistasis (p<0.01). 2. In the combination ratios of 1 : 6 (group I) and 1 : 10 (group F), as compared with the PEP 0.1μg/ml alone group (group E), each of 2-, 3-, and 5-day groups showed siginificant genesistasis (p<0.01). 3. In the PEP 1.0μg/ml alone group (group J), as compared with the combination ratios of 1 : 3 (group H), 1 : 6 (group I), and 1 : 10 (group F), each of 2-, 3-, and 5-day groups showed siginificant genesistasis (p<0.01). 4. In the combination ratio of 1 : 10 (group F), as compared with the combination ratio of 1 : 6 (group I), each of 2-, and 5-day groups did not show siginificant genesistasis (p<0.01). 5. On the basis of the foregoing results, the combination ratios 1 : 6 (group I) and 1 : 10 (group F), as compared with the PEP 0.1μg/ml alone group (group E), enhanced antitumor effect several times. Since Ceph has natural killer activity and can reduce the side effects of PEP as a biological response modifier, the present experiment results gave considerable suggestions for the clinical aspect, although many further experimental studies need to be made.
九州歯科学会の論文
1994-12-25

培養癌細胞による抗癌剤の効果を高めるための実験的研究 : KB 細胞に対する Biscoclaurine alkaroid と 5-Fluorouracil, Peplomycin との併用効果

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