脛骨の骨基質形成に関する X 線的, 光顕的および超微形態的研究 : 高タンパク食の影響
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概要
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The amount of calcium intake is known to affect bone construction greatly at the growth stage. However, reports of the effect of protein intake on bone construction vary and the mechanism has not yet been clarified. For this study, rats in the growth stage corresponding to human infants were used and the effects of calcium intake and high protein food on the formation of bone matrix in the tibia were investigated. The results were as follows : 1. Radiological findings on bone density Averages of thickness were determined by converting bone density of the cortical bone to Al equivalent. Significant differences were found between the control group and high protein food group, and between the calcium-deficient food group and calcium-deficient food + high protein food group, with 5% and 1% significance level, respectively. The control group showed higher values. 2. Analytical findings of Ca and P on the X-ray microanalyzer As compared with the control group, the relative Ca quantitative ratio decreaed in the order of the control group, high protein food group, calcium-deficient food + high protein food group, and calcium-deficient food group. Relative P quantitative ratio decreased in the order of the high protein food group, control group, calcium-deficient food group, and calcium-deficient food + high protein food group. 3. Histopathological findings A. Contact microradiogram findings Although the high protein food group showed about the same findings as the control group, a decrease in bone lacunae was found in some. In the calcium-deficient food group, enlargement of bone marrow and marked decrease in bone quantity were found. In the calcium-deficient food + high protein food group, as compared with the calcium-deficient food group, large and small bone resorption images were seen in the whole area but an increase in bone quantity was seen. B. Light microscopic findings The high protein food group, as compared with the control group, showed about the same findings, but a decrease in the haversian system was seen in some. In the calcium-deficient food + high protein food group, as in the calcium-deficient food group, bone quantity decreased extensively, but an increase in young bone cells was found in the osteoid layer. As to bone constriction, active reconstruction was seen. 4. Scanning electron microscopic findings High protein food group : As compared with the control group, enlargment of the bone marrow was seen and many resorption surfaces were found, Furthermore, collagen fibers were abundant in findings. Calcium-deficient food + high protein food group : As compared with the calcium-deficient food group, resorption surfaces decreased in number and matrix by collagen fibers running in one direction was beginning to be seen, but still many sparse minute fibers were seen. One the basis of the foregoing findings, it is suggested that the high protein food group shows an effect of promoting bone construction during the time of calcium-deficient food.
- 九州歯科学会の論文
- 1994-10-25