ラット臼歯での生活歯髄切断に関する水酸化カルシウム法とホルモクレゾール法による治癒の比較 : 未分化間葉細胞の mitotic activity

概要

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The purpose of this research was to assess the role of undifferentiated mesenchymal cells after pulpotomy using calcium hydroxide method (CHM) and formocresol method (FCM) during the pulp healing process. Bilateral maxillary 1st molars of 25 male Wistar rats were employed for the investigation in this study. The rats were sacrificed for histological examination at 1, 3, 7, 14 and 28 days postoperatively. Three hours before sacrifice, vinblastine sulfate (0.3 mg/ 100 g b. w.) was injected into the abdominal cavity for arresting the mitotic undifferentiated mesenchymal cells (MUM cells) of the residual pulp in metaphase. The MUM cells were calculated and statistically evaluated. Both CHM and FCM reached a maximum peak of the number of MUM cells at 3 rd day. When CHM and FCM were compared from 14 th day to 28 th day, the former showed that the significant (p< 0.05) reduction of MUM cells was more rapid and the number of MUM cells was relatively lower throughout the residual pulp. Residual inflammation at the wound surface and incomplete closure by osteodentin were found in FCM at the end of this study. These observations demonstrated that "Inflammation stimulates the proliferation of MUM cells and influcences morphological differentiation of MUM cells". Thus, the following conclusion can be made : Continual inflammation will give rise to a delayed healing of formocresol pulpotomy.
1994-02-25