破骨細胞の分化誘導活性に関する実験的研究
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概要
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In tooth movement, bone resorption was believed to be regulated by local chemical mediators, electric potential, etc. The bone is resorbed by osteoclasts of which function is controled by two mechanisms ; first is differentiation of osteoclasts and the second is activation of the cells. In the study of osteoclast differentiation, bone marrow culture is widely used in these days. The purpose of this investigation was to reveal the presence of the osteoclast inducing activity in the bone extract from alveolar bone of normal rats using mouse bone marrow culture, and to measure the intensity of the inducing activity during tooth movement. The results were as follows : 1. When the bone extract from alveolar bone of normal rats was added to mouse bone marrow cell and cultured for 7 days or more, osteoclasts were induced in the culture assessed by the occurrence of osteoclast specific function and antigen ; bone resorption and calcitonin receptors. 2. The osteoclast inducing activity in the extract greatly decreased after the treatment of repeated freezing and thawing. The activity in the bone extract was unstable after treating at 4℃ for 1 week. Contrary to the low temperature, the activity in the bone extract was stable at 56℃ for 30 min and tending to increase. 3. The activity has disappeared after dialysis, and the activity passed through ultra filtrating membrane with 10 kDa of cut off. 4. Osteoclast inducing activity greatly decreased in the case of alveolar bone extract prepared from the rats which were applied 10 g or 100 g force for 3 days or more at upper incisors, compared with that in the extract from untreated rats. 5. Osteoclast inducing activity in the extract from rats applied 10 g or 100 g force was smaller than that from the rats applied 0 g force vehicle. As stated above, it was shown that the bone extract from alveolar bone induced osteoclast differentiation in mouse bone marrow culture. It seems that the factor (s) which induce osteoclast differentiation, differes from the known hormones and local bone resorbing stimulators. In the rat applied mechanical force at upper incisors, inducing activity of osteoclast differentiation was reduced. This result may reflect the net decrease in osteoclast formation in alveolar bone which is indirectly affected by tooth moving force.
- 九州歯科学会の論文
- 1993-02-25