ラット臼歯での生活歯髄切断後の神経, および血管成分の mitotic activity の推移 : 水酸化カルシウム法と FC 法の比較
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概要
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Bilaterally pulpotomized wounds were made in the maxillary molar of 25 male Wistar rats to compare the effect on mitosis of neurovascular components with different pulp capping materials and methods (Ca(OH)_2 method : CHM and formocresol method : FCM). The rats were sacrificed at various time intervals (postoperative 1, 3, 7, 14 and 28th day) after pulptomy. Also, 3 hours before sacrifice, each animal was given an intraperitoneal injection of 0.3 mg/100 g b.w. of vinblastine sulphate. For histological investigation, serial sections at 8μm were made and stained with hematoxylin and eosin, or only with hematoxylin. All of the mitotic cells arrested in metaphase of neuronal (Schwann cell and fibroblast) and vascular (endothelial cell, smooth muscle cell and pericyte) components in the residual pulp tissue at various time intervals were calculated and evaluated. The results were as follows : 1. The maximum peak on mitotic activity of neurovascular components in the residual pulp was the 3rd day postoperatively in both CHM and FCM throughout the experimental period. 2. The mitotic activity since the postoperative 3rd day decreased faster by CHM than by FCM. 3. The healing process on mitotic activity of neurovascular tissue by CHM seems to be ended, but not by FCM on the postoperative 14th day. 4. The mitotic activity by FCM increased slightly on the postoperative 28th day. The derivating site for regeneration of neurovascular tissue shifted more apically. These chronological differences on mitotic activity of neurovascular components in the residual pulp by both CHM and FCM applied depend on different volume and quality of the necrotic tissue produced by the capping material and method, and the long-standing inflammation in the residual pulp tissue by FCM. The mitosis-arresting method in this study is available one for investigating the effect of various type of pulp capping materials and methods.
- 九州歯科学会の論文
- 1992-10-25