歯肉溝液中のアルカリホスファターゼの由来に関する研究
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概要
- 論文の詳細を見る
Various kinds of diagnostic parameters for periodontitis have been proposed and especially the parameters in gingival crevicular fluid (GCF) have been paid attention of clinical interest in recent years. Alkaline phosphatase (ALPase) activity in GCF is one of those parameters and the activity has been reported to be associated with the progression of periodontitis. Specific gram-negative bacteria which are thought to be etiology and pathogenesis of human periodontitis have been reported to show high ALPase activity. Although ALPase activity in GCF seems to be related to periodontopathic bacteria, previous reports have not shown quantitative comparison of ALPase activity between periodontopathic bacteria and other oral bacteria. Besides the activity might originate from host response against the inflammation. To clarify this, the effect of SDS on each ALPase of periodontopathic bacteria and mammalian species was examined. Furthermore, ALPase in GCF taken from some patients with adult periodontitis was analyzed. The results were as follows : 1. ALPase activity was detected in only 3 kinds of periodontopathic bacteria, A. actinomycetemcomitans, P. gingivalis and P. intermedia and no detectable activity was observed in other oral bacteria. Of 3 kinds of bacteria, P. gingivalis and P. intermedia showed extremely high ALPase activity and the activity of A. actinomycetemcomitans was less than a tenth activity of P. gingivalis or P. intermedia. 2. All ALPases of periodontopathic bacteria were inhibited strongly by 1% SDS. In contrast, 3 types of mammalian ALPases, liver/kidney/bone type, intestinal type and placental type, were not at all inhibited in the presence of 1% SDS. 3. ALPase activity in GCF from the patients consisted of combination of SDS-sensitive and SDS-resistant activities in multiple ratio of two types of the activity. 4. ALPase activity ratio (ALPase activity in the presence of 1% SDS / that in the absence of 1% SDS) in GCF showed a positive relationship with ALPase activity in the absence of 1% SDS in GCF. These results suggest that although ALPase assay in GCF has been proposed for estimation of periodontitis progress, the assay in combination with SDS is useful for establishment of qualitative classification of periodontitis.
- 九州歯科学会の論文
- 1992-10-25