Streptococcus mutans e 血清型菌に対するモノクローナル抗体に関する研究
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概要
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Monoclonal antibody-secreting hybridomas were produced by fusing myeloma cells (SP2/0-Agl4) with spleen cells taken from mice (BALB/c) which had been immunized with Streptococcus mutans (serotype e) whole cells. Antigen was extracted by autoclaving the cells of S. mutans (serotype e) in saline solution and purified on DEAE-Sephadex A-25, Sephadex G-200 and CM-Sephadex C-25 columns. The purified polysaccharide antigen consisted of rhamnose (69.6%) and glucose (30.4%) when determined by gas chromatography. Nine monoclonal antibodies reacting with the serotype e strains of S. mutans were examined immunologically for their specificity against crude and purified polysaccharide antigen preparations of the strains and whole cells of various members of the mutans group of streptococci. The immunological methods included immunodiffusion in gel, quantitative precipitin reactions, radioimmunoassay and enzyme immunoassay. Two monoclonal antibodies, S3-9 and S3-17 reacted in these immunological reactions not only with whole cells of serotype e strains of S. mutans but also with the antigen preparations from these strains. It was revealed in competitive quantitative precipitin reactions that among varying haptenic sugars tested beta-methyl-D-glucopyranoside and cellobiose had a marked inhibitory effect on the reactions whereas maltose did not have the effect. It was also shown by enzyme immunoassay that monoclonal antibody S3-9 did not react with whole cells of four strains of S. mitis, two strains of S. salivarius and four strains of S. sanguis. These results indicate that the beta-glucosyl moiety of the type e polysaccharide is the predominant antigenic determinant and that monoclonal antibodies are very useful for the analysis of streptococcal polysaccharide antigens and serological identification of the mutans group of streptococci.
- 九州歯科学会の論文
- 1992-08-25
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関連論文
- Streptococcus mutans e 血清型菌に対するモノクローナル抗体に関する研究 : 主論文の要旨
- Streptococcus mutans e 血清型菌に対するモノクローナル抗体に関する研究