ラット脳内セロトニン神経活動への拘束ストレスの影響に関する研究 : In vivo Microdialysis による検討
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概要
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Using the technique of in vivo microdialysis, extracellular concentrations of serotonin and 5-hydroxyindoleacetic acid (5-HIAA) were determined in the striatum, thalamus, dentate gyrus and prefrontal cortex while Sprague-Dawley rats underwent immobilization stress for twenty minutes. Microdialysis probes had semipermeable cellulose tubes (molecular weight cutoff 50, 000 ; O.D. 0.22 mm). Perfusion was started at 12 or 36 hours after implantation of the microdialysis probe under a freely-moving condition. Measurements of the extracellular concentration of serotonin and 5-HIAA were made at twenty minute intervals, which were started 120 minutes after the onset of the perfusion. Serotonin and 5-HIAA were quantified by a high-performance liquid chromatography coupled with an electrochemical detector. Extracellular serotonin levels increased following twenty minute immobilization stress in all four regions of the rat brain. The immobilization stress relatively increased the extracellular serotonin levels in the prefrontal cortex and dentate gyrus. These results suggest that twenty minute immobilization stress induces the activation of serotonin release in rat brain regions concerning the induction of anxiety.
- 九州歯科学会の論文
- 1992-02-25