ビタミン D の成長期下顎頭に及ぼす影響に関する実験的研究
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概要
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Vitamin D is an important factor for adjusting remodeling of bone tissue. Its effect on the mandibular condyle however has not yet been clarified. Three-week-old male rats of Wistar strain, therefore, were used and its effect on the mandibular condyle and tibia at growth stage was investigated. The results were as follows : 1. Radiological findings The mandibular condyle, tibia epiphysis, and tibia metaphysis in the vitamin D (VD)-deficient group and calcium and vitamin D (Ca・VD)-deficient group showed bone layers clearly on the whole, and their trabeculae were running densely and increased, as compared with those in the calcium (Ca)-deficient group. The Ca・VD-deficient group showed unclear bone layers and decrease in trabeculae as compared with the VD-deficient group. 2. X-ray microanalytical findings Relative Ca quantitative ratio (Ca/[Ca]c) and P quantitative ratio (P/[P]c), as compared with the control, were determined analysis by point-by-point quantitative analysis of Ca and P from each data. The values of Ca and P lowered in the order of the standard diet control group, VD-deficient group, Ca・VD-deficient group, and Ca-deficient group. 3. Histopathologic findings 1) Contact microradiogram findings i) As compared with those in the standard diet control group, the mandibular condyle and tibia metaphysis in the Ca-deficient group showed decrease in granular trabeculae in primary spongiosa and diminution of trabeculae in secondary spongiosa. The VD-deficient group showed decrease in trabeculae in primary spongiosa and secondary spongiosa. The Ca・VD-deficient group showed irregularity and uneven arrangement of trabeculae in secondary spongiosa. ii) As compared with that in the standard diet control group, the tibia epiphysis showed about the same findings in the VD-deficient group, and unclear granular trabeculae in primary spongiosa and thin and irregularly arranged trabeculae in secondary spongiosa in the Ca-deficient group and Ca・VD-deficient group. 2) General tissue findings i) As compared with those in the standard diet control group, the mandibular condyle and tibia epiphysis in the Ca-deficient group and Ca・VD-deficient group showed decrease in proliferative condrocytes, increase in hypertrophyic chondrocytes, small cell body and deformation of the nucleus, and decrease in calcified matrix. The VD-deficient group showed small hypertrophyic chondrocytes and thin trabeculae. ii) As compared with that in the standard diet control group, the tibia metaphysis in the Ca-deficient group and Ca・VD-deficient group showed increase in hypertrophic chondrocytes and decrease in trabeculae. The VD-deficient group showed about the same findings as the control group except for slightly thin trabeculae. 4. Electron microscope findings 1) Scanning electron microscope findings As compared with that in the standard diet control group, the mandibular condyle in the Ca-deficient group showed irregular cartilage layers and subchondrocyte bone layers, much incomplete longitudinal matrix in the walls of cartilage lacunae, decrease in trabeculae, abundance of networks of collagen fibrils, and many interstice. The VD-deficient group showed orderly cartilage layers and subchondrocyte bone layers as the standard diet control group. Although the walls of cartilage lacunae were surrounded by longitudinal matrix, trabecular were incomplete in some places. The Ca・VD-deficient group showed cartilage lacunae comparatively clearly and increase in formation of trabeculae as compared with the Ca-deficient group. 2) Transmission electron microscope findings As compared with those in the standard diet control group, osteoclasts increased and ruffled border was well developed in the Ca-deficient group. In the Ca・VD-deficient group, osteoclasts decreased and ruffled border was poorly developed. The VD-deficient group showed about the same findings as the standard diet control group. As compared with the standard diet control group, Ca-deficient group and VD-deficient group showed no changes in cell organellae in osteoblasts. In the Ca-deficient group and Ca・VD-deficient group, however, osteoblasts decreased and mitochondria tended to swell. On the basis of the foregoing findings, the effect of vitamin D on the mandibular condyle, tibia epiphysis, and tibia metaphysis was strongest in the Ca-deficient group, followed by Ca・VD-deficient group, and VD-deficient group.
- 1990-02-25
著者
関連論文
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