成長期顎骨の実験的骨粗鬆症に対する活性型ビタミン D_3 の影響に関する研究 : とくに歯槽骨の変化
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概要
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Three-week-old male rats of wistar strain were given a calcium-deficient diet and experimental osteoporosis was induced in them. These rats were then given 1α-OH-D_3 orally (0.05μg/kg and 0.1μg/kg), an analogue of active type vitamin D_3, three times a week. The rats were grouped into normal diet control group (control group), calcium-deficient diet group (deficient group), calcium-deficient diet with 0.05D_3 group (0.05D_3 group) and calcium-deficient diet with 0.1D_3 group (0.1D_3 group). The effect on the alveolar bone and tibia was investigated for weight and radiologically, photographically, photo-densitometrically, x-ray microanalytically, histopathologically, scanningelectron-microscopically, and hemologically. The results were as follows : 1. The body weight showed more increase, though slight, for 0.05D_3 and 0.1D_3 groups than for deficient group, in comparison with control group. 2. Light-microscopically, the densitometric curves of the alveolar bone for 0.05D_3 and 0.1D_3 groups were lower than those for deficient group. The widths of interosseous cortex and medial cortex of the tibia were about the same as those for deficient group. However, the densitometric curves of patterns of arrangement of trabeculae were irregular. 3. Photo-densitometrically, the bone densities of the alveolar bone and tibia were lower for 0.05D_3 and 0.1D_3 groups than those for deficient group. 4. X-ray microanalytically, the relative ratios of Ca and P by quantitative analysis to those of control group showed that Ca in the alveolar bone was higher for 0.05D_3 and 0.1D_3 groups than for deficient group. The relative ratios of P were high for 0.05D_3 group, Deficient group, and 0.1D_3 group, in descending order. In the tibia, the relative ratios of Ca were higher for 0.05D_3 and 0.1D_3 groups than for deficient group. The relative ratios of P were higher for 0.05D_3 group and deficient group than for 0.1D_3 group. 5. Histopathologically, contact microradiogram (CMR) findings in the alveolar bone showed more increase in thickness of the alveolar bone and more increase in resorption lacunae and Haversian system for 0.05D_3 and 0.1D_3 groups than for deficient groups. General tissue findings in alveolar bone showed increase in osseous layers in the alveolar bone, haversian canals, bone cavities, resorption lacunae, bone marrow, osteoclasts, and hypocalcification layers. Diminution of trabeculae was seen. Scanning-electron-micros-copically, osseous layers were incompletely formed and osseous layers on the side of the alveolar crest were formed wider in 0.1D_3 group than in deficient group and 0.05D_3 group, and the resorption lacunae were many and shallow. In the tibia, CMR findings and general tissue findings were similar to those in the alveolar bone. 6. Hemologically, electrolytic serum Ca and the ratios between serums Ca and IP in 0.05D_3 and 0.1D_3 groups, and serums IP and Cl in 0.1D_3 group were low. Biochemical AL-P, GOT and GPT were high in the examination of endocrine functions, N-PTH and CT were high and C-PTH was low.
- 九州歯科学会の論文
- 1988-12-25