実験的骨粗鬆症に対するカルチトニンの効果に関する研究 : とくに歯槽骨の変化

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Three-week-old male rats of Wistar strain were given calcium-deficient diet for four weeks and experimental osteoporosis was induced. The effect of eel calcitonin (ECT) (0.2u/kg intramuscular injection) on the alveolar bone and tibia was investigated for weight and photo-density, and radiologically, optically, X-ray microanalytically, histopathologically, scanning-electron-microscopically, and hemologically. The rats were grouped into normal-diet controls, normal-diet ECT group, calcium-deficient-diet group, and calcium-deficient-diet ECT group. The results were as follows : 1. Calcium-deficient-diet ECT group showed more increase in body weight than calcium-deficient-diet group, although there was some difference as compared with normaldiet controls. 2. Radiologically, osseous layers were thicker and trabeculae increased more and became more compact in the alveolar bone in calcium-deficient-diet ECT group than in calcium-deficient-diet group. In the tibia, interosseous cortex and medial cortex became thicker and trabeculae also increased. 3. Optically, the densitometric curves of the alveolar bone became higher, although in an instable pattern, in calcium-deficient-diet ECT group than in calcium-deficient-diet group. The densitometric curves of interosseous cortex and medial cortex in the tibia also became high with acute peaks. 4. In photo-density, the bone density of the alveolar bone showed higher results in calcium-deficient-diet ECT group than in calcium-deficient-diet group. The degree of increase in bone density was greater than the difference between normal-diet ECT group and normal-diet controls. In the tibia also, the bone density showed high results. 5. On the X-ray microanalyzer, the peaks of Ca and P in the alveolar bone were higher in calcium-deficient-diet ECT group than in calcium-deficient-diet group. In quantitative analysis by point-by-point analysis also, the relative Ca ratio (Ca/[Ca]c) and P ratio (P/[P]c), as compared with controls, were higher in calcium-deficient-diet ECT group than in calcium-deficient-diet group. Similar results were also shown for the tibia. 6. Histopathologically, findings of CMR images of the alveolar bone showed greater distributions of Haversian canals and bone lacunae, and decrease in resorption lacunae in calcium-deficient-diet ECT group than in calcium-deficient-diet group. In the general tissue findings in the alveolar bone, osseous layers were thick and Haversian canals, bone lacunae, and low-calcification layers were increased and arranged regularly. On the scanning electron microscope, osseous layers were thin and marrow cavities were large and sparse inside as compared with normal-diet controls, but marrow cavities were fewer as compared with calcium-deficient-diet group. Also, the osseous layers on the sides of the periodontium and alveolar crest were thickened. In magnification of surface of the osseous layer, calcified minute granular structures assembled compactly. In the tibia, CMR image findings and general tissue findings in calcium deficient-diet ECT group were about the same as those for the alveolar bone. On the scanning electron microscope, formation of osseous layers on the whole was poorer than that in normaldiet controls, but their thickness was incressed as compared with calcium-deficient-diet group. The margins of medial cortex were still irregular. In calcified areas, gaps in the calcification front were noticeable. 7. Hemologically, electrlytic serum Ca and the ratio between serum Ca and serum Ip were low in value in calcium-deficient-diet ECT group. The values of serum Ip, serum k and serum Cl were hihg. The values of biochemical GOT and GPT were hihg. In examination of endocrinic functions, CT and 1.25 (OH)_2 D_3 were high and C-PTH and 24.25 (OH)_2 D_3 were low. On the basis of the foregoing findings, the administration of calcitonin proved to have the effects of inhibition of bone resorption and acceleration of calcification and bone formation not only for the normal bone tissue but also for debilitated alveolar bone and tibia. However, for ameliorating bone debility due to insufficient intake of calcium, administration of calcitonin alone is considered inadequate and a combined diet with food high in calcium is considered necessary.
九州歯科学会の論文
1988-10-25

実験的骨粗鬆症に対するカルチトニンの効果に関する研究 : とくに歯槽骨の変化

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