免疫マウスにおける T 細胞のサブクラスの分布に関する実験的研究
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概要
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A possibility, that T lymphocytes are controled in their function by a modification of surface receptor through the binding of fluid factor in periodontitis, was studied. Both LPS immunized mice and non-immunized ones were injected by LPS in gingiva, and lymphocytes were prepared from submandibular lymph nodes and spleen with time after injection. Proportions of T lymphocyte subclasses were determined both by cytotoxic test and by fluorescent antibody technique, and also antibody titers to LPS were determined. The results obtained were as follows : 1. Antibody titers in mice either immunized intravenously (iv group) or immunized intraperitoneally (ip group) were 1024 fold just before injection in gingiva. 2. In ip group the titers after 1, 3, 5 and 7 days of gingival injection were 256, 1024, 512 and 512 fold respectively. In iv group those after 1, 3, 5 and 7 days were 256, 512, 256 and 256 fold respectively. The titers of ip group were relatively higher than those of iv group. 3. In immunized mice without gingival injection, ratios of suppressor T cell to helper one (Ts/Th) in spleen were 1.6 in ip group and 2.9 in iv group. Those in submandibular lymph nodes were 1.9 in ip group and 1.8 in iv group. Supressor T cell was dominant in number in both groups. 4. In ip group, Ts/Th's in spleen after 1, 3, 5 and 7 days of gingival injection were 1.8, 2.3, 1.0 and 2.1, respectively. Ts/Th's in submadibular lymph nodes of the same group after 1, 3, 5 and 7 days were 1.6, 1.9, 1.6 and 2.3 respectively. In iv group, Ts/Th's in spleen after 1, 3, 5 and 7 days were 1.9, 1.6, 1.3 and 1.7, respectively. Ts/Th's in submandibular lymph nodes after 1, 3, 5 and 7 days were 1.7, 1.7, 1.6 and 2.3 respectively. 5. In submandibular lymph nodes of ip group, ranges of proportion of IgG positive T cell after 1, 3, 5 and 7 days of gingival injection were 29-39%, 49-64%, 53-55% and 53-55% respectively. These values were relatively greater than that in control, 29-38%. In iv group these ranges after 1, 3, 5 and 7 days were 31-41%, 46-57%, 41-46% and 36-42% respectively. These values were little different from that in control, 37-47%. In spleen of ip group these ranges after 1, 3, 5 and 7 days were 21-34%, 36-45%, 27-39% and 21-35% respectively. These values were relatively greater than that in control, 19-27%. In spleen of iv group these ranges after 1, 3, 5 and 7 days were 22-30%, 38-56%, 37-46% and 36-47% respectively. These values were relatively greater than that in control, 15-29%. 6. Submandibular lymph nodes and spleen of immunized mice proportions of both suppressor T cell and IgG possitive one were greater than those of non-immunized mice. It was discussed that T cell in immunized mice was to be activated and/or differentiated through binding with IgG and the binding might play an important role in immunological response and control.
- 九州歯科学会の論文
- 1986-10-25