象牙質齲蝕における C. albicans の役割について
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概要
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In order to further clarify Candida albicans' (C. albicans) role in the development of dentine caries, research was conducted in the following areas : population of the organism in oral cavities, the organism's ability to use dentine powder for its growth, and the degradation of dentine collagen by an enzyme prepared from culture media. The results obtained are as follows : 1. One hundred and sixteen plaque samples and soft dentine samples were collected from caries carriers. One hundred and twenty-three plaque samples were collected from non-caries carriers. C. albicans was isolated from 37.9% of the soft dentine samples, and 26.7% from the dental plaque samples of caries carriers. It was also isolated from 14.6% of the dental plaque samples from non-caries carriers. 2. Growth of the C. albicans ATCC 1002 strain (1002 strain) was observed when the organisms were cultivated at 37℃ for 5 days in media containing only dentine powder and distilled water. The maximum growth was 6.0×10^3 CFU/ml, and was ten times greater than that growth obtained in water only (the control). Under the candle method, the maximum growth in the dentine powder media was 4.5×10^4 CFU/ml, and was again greater than that growth obtained in the water-only media using the same method. 3. It was observed that the 1002 strain could also grow in water containing dentine powder that was demineralized with 3% EDTA. Growth was the same as that obtained in the water containing ordinary dentine powder. 4. In fermentation tests, the 1002 strain produced acid and gas from seven sugars including glucose. The strain produced acid only from arabinose, but did not ferment raffinose and lactose. 5. Under the candle method, in both the ordinary and the demineralized dentine powder media, the growth of the 1002 strain was stimulated by the addition of glucose. The growth was about ten times greater than that obtained in the same media without the addition of glucose. 6. An enzyme which could degrade collagen was prepared from the supernate when the 1002 strain was cultivated in Yeast Carbon Base (YCB) containing demineralized dentine. It was shown that the enzyme degraded demineralized dentine, and released hydroxyproline into the solution. The enzyme also degraded Azocoll used as substrate. 7. The optimum pH in the lytic activity of the enzyme was 4.0, and the optimum temperature was 45℃.
- 九州歯科学会の論文
- 1985-10-25