培養正常細胞に対する諸種抗炎症剤の影響に関する研究

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Anti-inflammatory drugs used in the daily dental clinical work are the most frequently used drugs along with antibiotics. For evaluation of the effect of drugs, various methods have been reported to date. The tissue culture method is one of them and is an excellent one. The author cultivated in vitro the skin tissue of albino rat fetus and investigated the effect on the cultured cells of steroid anti-inflammatory drugs (SAID)(Predonine : Prednisolone sodium hemisuccinate, and Decadron : Dexamethasone sodium phosphate) and of non-steroid anti-inflammatory drugs (NSAID)(Solantal : Tiaramide hydrochloride, and Urokinase). The results were as follows : 1. In the treatment with SAID (Predonine and Decadron), proliferation of fibroblastilke cells was restricted, when the culture was treated after cultivation, with high dose of Predonine, 0.5mcg/ml, and Decadron, 0.02mcg/ml. In low dose treatment with Predonine, 0.005mcg/ml and Decadron, 0.0002mcg/ml, stimulatory proliferation suggestive of the effect of the drugs was observed in the fibroblast-like cells. When these drugs were applied simultaneously with cultivation, significant restriction of proliferation of the fibroblast-like cells was clearly observed. Morphological regressive changes in the cytoplasm, nucleus, and nucleolus were observed in both the cultured epithelium-like cells and fibroblast-like cells. These regressive changes were observed not only in the fibroblast-like cells but also in the epithelium-like cells. Furthermore, these changes intensified more in the high dose application group than in the low dose application group and in proportion to the number of application days. 2. In the treatment with NSAID (Solantal and Urokinase), restriction of proliferation of fibroblast-like cells was observed when high dose of Solantal, 5mcg/ml, and Urokinase, 0.06 unit/ml, was applied after cultivation. In the low dose treatment with Solantal, 0.05mcg/ml, and Urokinase, 0.0006 unit/ml, proliferation suggestive of the effect of the drugs was observed. When these drugs were applied simultaneously with cultivation, proliferation of fibroblast-like cells was more distinct. Morphological regressive changes were clearly observed in both the epithelium-like cells and fibroblast-like cells. These regressive changes were intensified in the high dose application group and in proportion to the number of application days. 3. No significant difference by drug was observed in restriction or promotion of proliferation, and in regressive changes in both cells. However, appearance of giantcell-like cells was observed in the experiment with SAID but not with NSAID. With Urokinase which is anti-inflammatory enzyme, regressive changes were observed in the epithelium-like cells rather than in the fibroblast-like cells.
九州歯科学会の論文
1984-02-25

培養正常細胞に対する諸種抗炎症剤の影響に関する研究

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