Tween 80 加培地における Candida 属の白輪形成ならびにそれの菌種同定への応用について

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概要

• 論文の詳細を見る

• Martin's classification is currently favored for Genus Candida which depends chiefly on the modes of chlamydospore formation and sugar fermentation. The author undertook a series of study on the modes of white ring formation of the Genus candida by use of SCLT medium (Akisada) and PT medium (Akisada), of which the former has recently been proved very efficient in promoting the chlamydospore formation and the latter in promoting the white ring formation. The composition of the former medium is as follows. soluble starch 10 g corn meal extract 330 ml liver extract (300 g/500 ml) 40 ml Tween 80 8 ml agar 20 g distilled water 630 ml Adjusted to pH 7.4 with NH_3 The composition of the 2nd medium is as follows. peptone 10 g Tween 80 8 ml agar 20 g distilled water 1000 ml Adjusted to pH 6.6 It was noticed with use of these two media, that some species of Candida produced white ring around periphery of colonies and others did not. The present study concerned with the detailed cultural conditions and possible mechanisms to explain the phenomenon, and results were summarized as follows. 1. Of seven species of genus Candida, C. albicans, C. tropicalis and C. stellatoidea produced the white ring, while C. pseudotropicalis, C. krusei, C. parakrusei and C. guilliermondii failed to do so. This would seem to provide a convenient method for the preliminary differentiation of the organisms. 2. Of the ring-forming organisms, C. tropicalis produced the strongest white ring, while C. stellatoidea and C. albicans produced weaker ones. Of the latter two, C. stellatoidea produced the ring more distinct than the one produced by C. albicans. 3. The optimum conditions for the ring formation were first to use the fresh culture of the organisms on Sabouraud glucose agar maintained for 24 to 48 hours at 37 C. The optimum pH was 7.4 for SCLT medium and 6.6 for PT medium and 25 C was required for optimum growth in these media. 4. When Tween 40, Tween 20, lard oil, olive oil were used respectively instead of Tween 80, similar ring formation was observed. 5. When Tween 80 and other fatty substances were removed from the medium, the ring formation was never observed. 6. When sucrose was added to SCLT and PT media to see its effect on the ring formation, C. albicans and C. tropicalis failed to produce the white ring by sucrose consentrations of 0.5 % in the former and 0.8 % in the latter, while no inhibition of ring formation was observed with C. stellatoidea at the concentration of 1.0 %. Evidences showed that the inhibitory effect of sucrose addition derived from lowered pH of culture medium by sucrose addition rather than any direct action of sucrose itself. 7. A possible mechanism for the white ring formation was regarded to be the activity of lipase which is present in-and outsides of these three species of Candida.

• 九州歯科学会の論文
• 1973-03-31

著者

• 北村 吉緒

  九州歯科大学細菌学教室

• 北村 吉緒

  九州歯科大学保存学教室

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