Expression of Cell Proliferation-associated Nuclear Antigen (Ki-67) in Recurrent Aphthous Ulcers (RAU)

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The histological features of recurrent aphthous ulcer (RAU) are consistent with increased number and activation of T cells in the diseased areas. A previous study suggested that 13-23% of the cells express IL-2 and transferrin receptors, which implies potential for local proliferation. The aim of the study was to to evaluate the proliferation activity in 10 RAU lesions compared to 9 oral traumatic ulcer (TU) lesions. Demonstration of proliferating cells was performed by application of affinity-purified rabbit anti-human Ki-67 antibodies in avidin-biotin-peroxidase complex (ABC) staining to both RAU and TU biopsies. We found that the basdsuprabasal layers of RAU epithelium away from the ulcer contained 30% more proliferating cells (127±24 vs 97±19 cells/0.2 mm^2, P<0.01) than the epithelium of TU. Furthermore, 9±4% (115±29 cells/0.2 mm^2) of the infiltrating inflammatory cells in RAU lesions were Ki-67-positive proliferating cells, compared to only 3±1% (37±14 cells/0.2 mm^2) in TU inflammatory infiltrates. These findings suggested that the mitotic activity is increased in RAU epithelium and that the relatively longer persistence of RAU cannot be explained by a lack of new epithelial cell production. Furthermore, mononuclear inflammatory cells replication in situ represent one mechanism for the expansion of the inflammatory cell infiltrates at sites of RAU and TU lesions.
特定非営利活動法人日本臨床口腔病理学会の論文