心臓アレルギーにおける抗原の決定分子群としての心筋セファリンの有效分屑の吟味
スポンサーリンク
概要
- 論文の詳細を見る
A series of experiments of allergy in the laboratory of Prof. Maekawa proved that a factor which ordains allergy is not heterogeneous protein that acts as a carrier, but organ (or tissue) phosphatide that has organ (or tissue) specificity, but no group specificity. Further experiments confirmed that a determinant group of antigen in allergy is organ (or tissue) cephalin which belongs to organ (or tissue) phosphatide. Recently it was proved in Erythron-Allergy that the effective fractions of the cephalin are Fractions I and II extracted by means of Folch's chloroform-alcohol method.It is the purpose of the present experiment to decide, in which fraction from myocardium cephalin an effective component as an antigen in heart allergy presents, and whether lecithin fraction from myocardium cephalin has allergic antigenicity or not.Materials and Method By means of Araki's method, cephalin and lecithin were extracted from ox myocardium. Further fractions were isolated from the cephalin by means of Folch's chloroform-alcohol method.Antigens : -Group I : 2% refined egg albumin solution (2 cc/kg) plus Fractions I and II of cephalin from ox myocardium (1 mg/a rabbit)Group II : 2% refined egg albumin solution (2 cc/g) plus Fraction III of the same cephalin (1 mg/a rabbit)Group III : 2% refined egg albumin solution (2 cc/kg) plus Fraction V of the same cephalin (1 mg/a rabbit)Group IV : 2% refined egg albumin solution (2 cc/kg) plus lecithin fraction from ox myocardium (6 mg/a rabbit)Control group : 2% refined egg albumin solution (2 cc/kg)Rabbits were sensitized by intravenous injection of the above mentioned materials for 4 days (once a day), and examined Arthus phenomenon 3 weeks later.At the 7th day after reinjection, all rabbits were killed, and at once their hearts, kidneys, lungs, spleens and suprarenal bodies were removed and fixed by10% formalin, and their sections were cut in paraffin and stained by hematoxirin-eosine.Throughout this experiment, all rabbits were observed clinically, serologically and electrophoretically.Results 1) No remarkable changes were observed in their weights, temperatures and findings of blood. In urines of some rabbits, protein and urobilinogen were observed temporarily.2) In the Ecg., Groups I and II showed lower T, biphasic T and so on, after sensitization and reinjection. These findings show the appearance of myocardial injury. In Group IV and control group, no or slight changes were observed.3) In the serum protein, Groups I and II showed restriction of increase of γ-globulin after reinjection, and in one rabbit (No. 502), β_2-globulin appeared after reinjection. These findings show the appearance of allergy and that the allergic antibody adheres to tissue.4) Histological findinds : The changes in heart were generally interstitial myocarditis. The degree of the changes was most remarkable in Group I (all rabbits), and also in Groups II and III, a few rabbits showed myocarditis of slight degree. On the contrary, in Group IV and control group changes in heart were hardly observed. The degenerations of parenchym of myocardium were slight in general.Other organs showed no remarkable allergic changes.Summary and Conclusion 1) In heart allergy, as in Erythron-Allergy, the fractions of myocardium cephalin as a determinant group of its antigen are obviously Fractions I and II (by Folch), and it is supposed that the main component is inositol phosphatide from the results of analysis of brain cephalin by Folch. Moreover, also these fractions have organ specificity.2) The electrophoretic patterns of the serum protein show that the allergic antibody adheres to tissue.3) The lecithin fraction from myocardium phosphatide is undoubtedly no determinant group of the antigen also in heart allrgy.4) The more refindly egg albumin is prepared, the more difficult it becomes to adhere to cell phosphatide (or cell cephalin, or its further fractions), and so the allergic antigenicity of the mixture of them is weaker compared with that of the phosphatide and bovine serum mixture.
- 社団法人日本循環器学会の論文
- 1955-11-20