Peroxidase Activity, Isoenzymes and Histological Localisation in Sapwood and Heartwood of Scots Pine (Pinus sylvestris L.)

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概要

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• Peroxidase activity and isoenzymes of fresh wood samples of the third shoot of 12-year old trees and from the sapwood, transition zone and heartwood of c. 60-year old stems of Scots pine (Pinus sylvestris L.) were investigated. Wood samples were ground at -30℃, extracted, and the extracts concentrated c. 20-fold for peroxidase activity assays (guaiacol method) and for IEF-PAGE. At least I I major isoenzymes could be found in the gels . Even the heartwood contained some peroxidase isoenzymes. Isoenzyme patterns of the juvenile wood did not change with the season. Howewer. juvenile wood showed the highest peroxidase activity at the end of the growing season. Peroxidase activity decreased from the outer sapwood towards the heartwood. Thin sections of different wood zones stained for peroxldase revealed activity in ray parenchyma and resin canal epithelial cells. Intensive staining was localised in the bordered pits of vertical and ray tracheids, and in the end walls of ray parenchyma cells.

• 一般社団法人日本森林学会の論文
• 1998-02-16

著者

• SARANPAA Pekka

  Finnish Forest Research Institute

• Fagerstedt Kurt

  Department of Biosciences, Division of Plant Physiology, University of Helsinki

• Piispanen Riikka

  Finnish Forest Research Institute

• Fagerstedt Kurt

  Department Of Biosciences Division Of Plant Physiology University Of Helsinki

関連論文

• Crystallinity of wood and the size of cellulose crystallites in Norway spruce (Picea abies)
• Microfibril angle of Norway spruce [Picea abies (L.) Karst.] compression wood: comparison of measuring techniques
• Peroxidase Activity, Isoenzymes and Histological Localisation in Sapwood and Heartwood of Scots Pine (Pinus sylvestris L.)

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