新規な機能をもつ核酸結合性小分子の研究
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概要
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DNA ligands carrying new functions such as metal binding capability and redox activity were synthesized. These novel DNA ligands were designed for 1) hydrolytic cleavage of DNA phosphodiester backbone assisted by the metal ion and 2) the ultrasensitive detection of DNA by using electrochemical methods. In the DNA cleavage experiments, a cooperativity was observed between the action of metal ions and metal-binding DNA intercalator. It was assumed that the strain in the phosphodiester bonds induced by the intercalator accelerated the metal-assisted hydrolysis reaction. This is the first example of the intercalator-metal cooperated hydrolysis of DNA phosphate chain. The electrochemical labeling of DNA based on reversible binding modes such as intercalation and oligonucleotide-directed triple helix formation was realized. Using the spectroscopic, hydrodynamic and voltammetric methods, the author has made it clear that a bisacridine-type DNA ligand containing viologen unit is useful as a nondestructive activating reagent for DNA for electrochemical study of DNA-ligand interactions. On the other hand, oligopyrimidines modified with ferrocenyl group as a reporter moiety have been proven to be applicable to practical use as a probe for electrochemical detection of targeted double stranded DNA. This detecting system combined with HPLC (equipped with an ordinary electrochemical detector) has facilitated an ultrasensitive analysis of DNA sequence ; detection below femto mol level has been accomplished. The methodologies developed in these studies provide a new possibility in analytical technique of DNA. In addition, a facile introduction of redox active site onto DNA is expected to give a new tool for studying the chemistry of DNA.
- 1994-02-05
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関連論文
- 新規な機能をもつ核酸結合性小分子の研究
- 高速液体クロマトグラフィーによる微量重金属の同時定量法に関する研究/新規な機能をもつ核酸結合性小分子の研究
- ストリット型プローブの協同的金属錯体形成を利用するDNA の認識及び検出 (特集 分離分析試薬の新展開)