バイオセンサーによる飲料水中のリン酸イオンの計測
スポンサーリンク
概要
- 論文の詳細を見る
Biosensors are expected to be simple, rapid, highly selective, and ecologically sound methods. We previously developed several phosphate ion (Pi) biosensors, the latest of which was a chemiluminescent-flow injection analysis (CL-FIA) system; however, its sensitivity and stability were not sufficient. As a result, it was found that, to implement our Pi biosensor, four conditions were required. These four conditions were: to achieve stability with high sensitivity (0.32 μM Pi; maximal permissible concentration in Japan); to establish a removal method of dissolved substances; to measure real samples; and to produce a complete automated biosensor system satisfying the above three requirements. To satisfy all requirements, we chose a biosensor using pyruvate oxidase G (from Aerococcus viridans) in three newly developed biosensors, and the biosensor was fitted compactly into a trial automatic CL-FIA system of the desktop type. After optimization, this system could detect 96-nM to 32-μM Pi (within 30 s) with an RSD of 2.3% (n=5) at 25±0.1℃ and make the calibration curves (0.16-μM to 32-μM Pi, r=1.00) for at least two weeks. Treatment with activated carbon could improve the sensor response when inhibited by dissolved substances in fleshwater. The results, determined Pi concentrartions in real samples, obtained by the sensor system and the modified molybdenum blue method were compared, and good correlation (r=0.94, n=7) was obtained between them.
- 社団法人日本分析化学会の論文
- 2001-08-05