キャピラリー電気泳動による光学活性医薬品の分離分析法に関する研究

概要

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Enantiomeric separation of chiral drugs was investigated by capillary electrophoresis (CE) with various chiral selectorS. When a protein was used as a chiral selector, UV absorption of the protein itself disturbed the detection of analytes. In order to overcome this drawback, a partial-filling technique was developed. A high protein concentration was used without causing low detection sensitivity. Various enantiomeric separations were successfully obtained using the partial-filling technique as well as several proteins, such as egg-white avidin and α_1-acid glycoprotein. Additionally, the change in enantioselectivity of avidin by the addition of D-biotin was investigated. On the other hand, several ionic cyclodextrin derivatives were effective as chiral selectors for various enantiomeric separations without the partial-filling technique. The analytes were classified into three groups according to the separation conditions, and the classification was useful for optimizing the separation conditions. The partial-filling technique was useful for enantiomeric separations by capillary electrophoresis/mass spectrometry (CE/MS) using proteins and cyclodextrins as chiral selectors. The introduction of nonvolatile chiral selectors into the MS instrument was diminished by this technique. These methods, CE and CE/MS, were applied for optical purity tests of chiral drugs and enantiomeric separation of chiral drugs in human plasma.
1999-11-05