MALDI-TOF質量分析法を用いた新しい糖鎖構造解析法

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概要

• 論文の詳細を見る

• Structural analyses of carbohydrates were performed by a new technique using MALDI-TOF mass spectrometry. Sequential fragment ions were observed in the post-source decay (PSD) fragment spectra of MALDI-TOFMS, which are like the MS/MS fragment spectra. These fragment ions were produced by cleavages of the glycosidic linkages. The structural isomers of sugar-branched cyclodextrin derivatives were distinguished by a relative ion intensity analysis. The fragment ions produced by one-site cleavage of the glycosidic linkage had a much higher intensity than that produced by two-site cleavage. In addition, the glycosidic linkage of α1-4 cleaved more easily than that of α1-6 in MALDI-PSD fragmentation. These results strongly indicated that the relative ion intensity analyses of these MALDI-PSD fragment ions enable us to distinguish structural isomers and to perform a linkage analysis.

• 社団法人日本分析化学会の論文
• 1999-10-05

著者

• 山垣 亮

  通商産業省工業技術院生命工学工業技術研究所生体物質部生体物質化学研究室

関連論文

• MALDI-TOF質量分析法を用いた新しい糖鎖構造解析法
• A new technique of carbohydrate analysis using post-source decay fragmentation by MALDI-TOFMS/Studies on the non-destructive of dyestuffs used in ancient colored cloths and ukiyo-e prints by the three-dimensional display of fluorescence spectra

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