固定化した生物物質による反応及び分離・検出を集積化した分析システムの開発

概要

論文の詳細を見る
Analytical systems that integrate biological reactions by immobilized biomolecules were developed. For the immobilization of diomolecules in a micro-sized flow-through system, a novel hiomolecule-encansulation technique was developed using a sol-gel reaction. Various biomolecules were encapsulated in a tetramethoxysilane (TMOS)-based hydrogel within a capillary in a single step under mild conditions. This monolithic column showed an adequate chromatographic performance, including mechanical strength, and penetration of pressurized flow. The encapsulation of bovine serum albumin and ovonlucoid in a TMOS-based matrix allowed for the separation of drug enantiomers. A trypsin-encapsulated capillary column enabled the creation of an on-line enzyme reactor with enhanced reactivity. Furthermore, by encapsulating microsomes isolated from rat liver, an on-line drug-metabolism analytical system was developed, where the metabolic reaction of drug and the determination of the metabolites were integrated in a single capillary. This thesis also presents the in situ fabrication of trypsin-encapsulated gels onto a microchip for an on-chip bioreactor, in which integrated protein digestion, separation, and detection were performed. This encapsulation technique opens a new pathway for the application to automated and high-throughput analytical schemes for a multi-analyte device or drug-screening platform.
社団法人日本分析化学会の論文
2004-10-05