ビタミンB_<12>要求性大腸菌変異株の研究 : (V)BACK MUTATIONの対策

概要

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A suitable method for the maintenance of the stock culture of a mutant strain of Escherichia coli was discussed. When a seed culture is required it is advisable to prepare and store a large number of simultaneous subcultures from one culture of #215,rather than to prepare by repeated serial transfers. Frequent transfers should be avoided. The culture is desirable to be stored in a dark room at a low temperature. When prototrophs are found in such a stock-culture, they can be eliminated as rapidly as possible by penicillin technique. The rescreened auxotrophs should be used as a test organism after being transfered one to two times in a medium containing methionine.
日本ビタミン学会の論文