シロネズミ肝Transketolase活性に及ぼすThiamine欠乏, その回復および2,3のホルモンの影響について

スポンサーリンク

概要

• 論文の詳細を見る

• Albino rats were made deficient by thiamine deprivation, restored by thiamine administration, loaded with a large dose of thiamine and made diabetic with alloxan or adrenalectomized. The transketolase activities and thiamine contents in the liver of these animals were determined and the following results were obtained. 1. Thiamine deficiency caused a marked decrease in rat liver transketolase which showed one-seventh of that for the control after 28 days of thiamine deprivation, though the activity increased tentatively in the beginning of deficiency. The thiamine content in the liver also decreased to one-tenth of that for the control. 2. The decreased activities could not be restored by the addition in vitro of thiamine diphosphate on the contrary of α-keto acid dehydrogenase in liver mitochndria. This suggested the decrease of apo-andor holoenzyme of transketolase. 3. In restoration of thiamine deficiency with thiamine (40 μg) administration, the transketolase activity was almost not improved after six hours of the administration, although thiamine content was recovered to normal. The complete restoration of the enzyme needed 3 to 5 days with daily administration of thiamine (40 μg), although daily administration of thiamine (2 mg) promoted the enzyme restoration. 4. Thiamine pyrophosphokinase in the liver had not altered through the deficient stadium. 5. Daily administration of a large dose of thiamine to rats gave no change in liver transketolase of which was observed no coenzyme induction with thiamine as well as other thiamine dependent enzymes. 6. Alloxan treatment or adrenalectomy and treatment with insulin or hydrocortisone of rats had no effects on the activities of liver transketolase.

• 日本ビタミン学会の論文
• 1971-11-25

著者

• 高橋 麒三郎

  京都府立医大生化学

• 高橋 麒三郎

  京都府立医科大学生化学教室

関連論文

• 6.大腸菌におけるビタミンB_1補酵素量によるTransketolaseの変動(一般研究発表要旨)
• 5.大腸菌のThiamine uptake-negative mutant(第180回会議研究発表要旨)
• 7.ネズミ肝Thiamine Diphosphate依存酵素に対するThiamine欠乏ならびにThiamine投与の影響(THE JOURNAL OF VITAMINOLOGY : 掲載論文要旨)
• シロネズミにおけるビタミンB_1投与量によるα-ケト酸酸化酵素の変動
• シロネズミ肝Transketolase活性に及ぼすThiamine欠乏, その回復および2,3のホルモンの影響について
• 大腸菌におけるThiamine含有量とTransketolase活性の変動について
• Transketolase測定法(Racker法)の検討
• 19.ビタミンB_1欠乏および2,3のホルモンのシロネズミ肝Transketolase活性におよぼす影響について(研究発表)(日本ビタミン学会 : 第23回大会研究発表要旨)
• 1.Transketolase測定法(Racker法)の検討(ビタミンB研究委員会 : 第202回会議研究発表要旨)
• 8. 大腸菌のビタミンB_1合成調節変異株によるHydroxymethylpyrimidineの蓄積(英文誌抄録 掲載論文要旨)

スポンサーリンク