ヒト赤血球およびその精製ヘモグロビンとビタミンB_1誘導体, とくにThiamine tetrahydrofurfuryldisulfideとの反応

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When thiamine tetrahydrofurfuryldisulfide (TTFD) and other thiamine disulfide derivatives were incubated with human erythrocyte, a detectable amount of free thiamine has been noted in the incubation mixture in addition to intracellular accumulation of thiamine. Results showed that hemoglobin has the major role of the reduction of thiamine disulfide derivatives to form free thiamine in erythrocytes. It was also confirmed that this free thiamine was converted in vitro to thiamine diphosphate in the presence of adenosine triphosphate by thiamine pyrophosphotransferase from pig heart. When native or denatured purified human hemoglobin was incubated with TTFD, the former required two times amount of the latter to form a certain amount of free thiamine at 37℃. The contents of active thiol were calculated to be 1.68 moles per mole of native hemoglobin and 3.2 moles per mole of denatured one by the titration with PCMB. Sephadex gel filtration studies of the incubation mixture with ^<35>S-labeled TTFD (outer) and hemoglobin or glutathione indicated the formation of several radioactive compounds. It was also noticed that the disulfide compound produced from TTFD with hemoglobin was more labile than that of glutathione. From these results it was presented that the possible reaction mechanism between thiol groups of hemoglobin and thiamine disulfide derivatives was as follows : α_2(-SH)_2 β_2(-SH)_4+2B_1-S-S-R ⇄ α_2(-SH)_2 β(-SH)_2(-S-S-R)_2+2B_1
日本ビタミン学会の論文
1967-06-25

ヒト赤血球およびその精製ヘモグロビンとビタミンB_1誘導体, とくにThiamine tetrahydrofurfuryldisulfideとの反応

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