大腸菌のビタミンB_1生合成にかんする研究 : (I)大腸菌洗滌菌体および菌体抽出酵素によるHydroxymethyl-pyrimidineおよびThiazoleよりのビタミンB_1生合成

概要

論文の詳細を見る
Thiamine biosynthesis from pyrimidine and thiazole moieties of thiamine by washed cells and cell extracts with sonification of several strains of Escherichia coli was investigated. E.coli ATCC 9637,70-17 and 26-43 which are thiamine requiring mutants derived from 9637,synthesized thiamine from OMP and Th, and the presence of OMP-kinase, Th-kinase and TMP synthetase that are involved in thiamine biosynthesis, was demonstrated. Only 70-23,strictly requires thiamine for its growth, failed to synthesize thiamine from OMP and Th, and no detectable OMP-kinase activity was found in the cell extract. The authors therefore supposed that thiamine requirement of this mutant based on the lack or decrease of OMP-kinase activity. strains 70-17 and 26-43,which were capable of growing in the presence of OMP or Th respectively, synthesized much more amount of thiamine than their parent strain both with washed cells and cell extracts.
日本ビタミン学会の論文
1963-08-25