Thiamine-8-(methyl 6-acetyldihydrothiocatate)disulfideにかんする研究 : (I)理化学的性質および定量法

概要

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Investigation was made on the physicochemical properties and quantitative determination of TATD. TATD is a white odorless, crystalline substance, practically insoluble in water, but TATD hydrochloride has a bitter taste and is very soluble in water. The ultraviolet absorption spectrum of its aqueous solution is variable with changes in the pH. Its isosbestic points are at 240,275 and 303 mμ. An aqueous solution of TATD is stable to heat in acidic enviroments. The powder of TATD is very stable even when mixed with lactose or precipitated calcium carbonate. TATD is reduced by sodium thiosulfate, cysteine, sodium hydrosulfite and zinc powder. The optimum condition of reduction was studied. TATD (1〜200μg) was reduced quantitatively with 10mg of sodium thiosulfate in a pH of 4〜5 at 40〜60℃ for 15 minutes. Thiamine thus produced was determined by the thiochome method. S-Acetyl radical was also determined by the formation of acetohydroxamic acid according to Lipmann and Tuttle.
日本ビタミン学会の論文
1963-05-25