ピリドキサール燐酸の製造に関する研究 : (I)濾紙クロマトグラフィーによるピリドキサール燐酸の定量

概要

論文の詳細を見る
The estimation of pyridoxalphosphate using paper partition chromatography was studied. As the developing solvent, ethylacetate・2,4-lutidine・ethanol・water (4 : 2 : 1 : 2) was selected because it gave good separation. The separated zone of pyridoxalphosphate was eluted with phosphate buffer (pH 7.0) and the quantity of pyridoxalphosphate was calculated from the extinction at 388 mμ. The experimental procedures were examined in details and the recovery of the experiment was 97% when pure pyridoxalphosphate was used. The coexistence of some of the hitherto known vitamin B_6 derivatives did not influence the value obtained.
日本ビタミン学会の論文