ビタミンB_2の組織化学的研究
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In the usual histochemical methed on riboflavin, it was difficult to observe free form and esterified form (FAD or FMN) separately. Moreover, since the fluorescence of FAD is not so much intensive as that of FMN or free riboflavin, measured amounts of FAD are always lower than the true values. Therefore, a trial for improvement of the histochemical method was carried out. Total riboflavin was estimated after hydrolysing the esterified forms by phosphatase, in tissue slice prepared by the author's procedure. Estimation of the esterified form was made after eliminating the free riboflavin by benzyl alcohol. The amount of the free form was calculated from the values of the total and esterified riboflavin. By this improved method, histochemical observation was done on the distribution of riboflavin in various organs of rats. In the small intestine, phosphorylation of riboflavin occurred in Golgi substance and basial mitochondria. FAD formation from free riboflavin or FMN was occurred mainly in the liver. Generally the riboflavin in the living bodies was present in the form of FAD.
- 日本ビタミン学会の論文
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