酵素的方法による植物葉肉遊離細胞およびプロトプラストの調製(細胞壁物質分解酵素シンポジウム)

概要

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Morphologically unimpaired mesophyll cells were isolated in large quantities by digesting stripped plant leaves with a crude polygalacturonase preparation from Rhizopus sp. The isolated cells were further treated with a crude cellulase preparation from Trichoderma viride to yield spherical protoplasts. Mesophyll cells or their protoplasts isolated from tobacco leaves could be maintained intact for several days as a suspension in a liquid medium. Active metabolism of the cells isolated by these procedures was indicated by the ability of tobacco protoplasts to synthesize tobacco mosaic virus upon infection with viral RNA. Some of the conditions of enzymatic treatments which affect the yield of intact cells are discussed.
社団法人日本生物工学会の論文
1969-05-25