Neurospora属菌によるセルラーゼ生産について : (第1報)培養条件および粗酵素の2,3の性質

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A strain of Neurospora species, isolated from a pile of Kihada-chips (Phellodendron amurence Ruprecht) treated with methanol, was found to produce a strong cellulolytic enzyme activity, especially on filter paper.Using this strain, the culture conditions for the production of cellulolytic enzyme in the culture filtrate and some properties of the crude enzyme were studied. Formation of filter paper degrading cellulase was induced effectively by the addition of native cellulose such as pulp powder. Ammonium sulfate was found not to be as suitable as defatted soy bean meal and corn steep liquor for the nitrogen source.A rather lower C/N ratio was required for the cellulase production.Crude Neurospora-cellulase had an optimum pH on filter paper degradation in the range of 4.5-6.0 at 40℃, and with an optimum temperature of about 50℃ within 90 min incubation.On heat-treatment at 60℃, pH 4.0,for 24 hr, about 45% of the Avicel saccharifying activity remained, while filter paper degrading activity entirely ceased.In the presence of Ag^+, Cu^<++>, Hg^<++> and Fe^<+++>, cellulase activities were inhibited. With carboxymethylcellulose as substrate, Michaelis' constant was found to be 1.07 (g/l) at pH 4.5 and 40℃.
社団法人日本生物工学会の論文
1967-04-25