ひいろたけ酵素の生産と応用 : (第5報) セルラーゼ系の分離精製 (その2)

概要

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In the preceding paper we reported the fractionation of the cellulolytic enzyme system of Trametes sanguinea into at least three cellulase components.The present papar concerns with the crystallization of one cellulase component, which is highly active on hydrocellulose, by means of DEAE-Sephadex column chromatography, ammonium solfate fractionation and the salting-out method.The crystalline enzyme was electrophoretically homogeneous and also homogeneous in the ultracentrifuge.The enzyme was highly active on insoluble cellulosic materials such as crystalline cellulose powder (Avicel) and hydrocellulose.When absorbent cotton was incubated with this enzyme, its native fibers were decomposed into tiny pieces, increasing gradually reducing power in the reaction mixture.The enzyme was nearly inactive against CMC and cellobiose.Some of the properties of the enzyme are summarized in Table 4.
社団法人日本生物工学会の論文
1965-09-25