イオン交換樹脂クロマトグラフィーによるロイシンおよびイソロイシンの分離

概要

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L-Leucine and L-isoleucine were completely separated on the column partition chromatography employing sulfonated polystyrene resines. L-Leucin, L-isoleucin and L-valine, each 1 mg, were chromatographed on the 100×0.9 cm column of Dowex 50-X4 (W type) of the particle size of 270〜330 mesh, with 2.0N hydrochloric acid for about 4 days at 22〜26℃. L-Valine, L-isoleucine and L-leucine were eluted in this order and a long interval was found between the fractions of isoleucine and leucine.Similar procedures were carried out on 75×1.1 cm column of Dowex 50-X8(200〜400 mesh, W type) with 0.1M citrate buffer, pH 4.25±0.05,containing n-propanol. The order of elution of amino acids was the same with that of X4 column, although the separation of leucine and isoleucine was not so satisfactory as compared with that of X4 column.
社団法人日本生物工学会の論文
1961-05-15