凝集性酵母の育種と育種株の発酵特性 : 凝集性酵母によるエタノール生産(第2報)

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To achieve high ethanol production using molasses as a raw material, we tried to construct flocculating yeasts from non-flocculating yeasts. An auxotrophic mutant, HM 1,with flocculating ability derived from a non-flocculating yeast, Saccharomyces cerevisae IFO 0224,was hybridized by protoplast fusion with an auxotrophic mutant, HM 2,of another non-flocculating yeast, S. cerevisiae EP 1. The fusant HF 1 showed not only good flocculation but also good ethanol production, as much as the parental strains. About 40% of fusants which showed good flocculation dispersed the flocculation during subcultures. But the flocculation of strains isolated from spores of good flocculating fusants remained stable, and good flocculating strains were isolated from spores of flocculating fusants with a high frequency of 60 to 80%.To enahance the flocculation of HS 1 which was isolated from spores of HF 1,an auxotrophic mutant, HM 4,of HS 1 was hybridized by protoplast fusion with an auxotrophic mutant, HM 3,of S. cerevisiae IFO 1953 which showed excellent flocculation.To construct good ethanol-tolerant strains, the fusant HF 2 between HM 3 and HM 4 was acclimated by repeating batch fermentation with molasses medium containing 22 w/v% total sugar at 33℃ and pH 5.0. The acclimatant HA 2 produced 95 g/l ethanol from molasses medium containing 25 w/v% total sugar at 35℃ and pH 5.0.HA 2 was tested in batch fermentation for the effects of (NH_4)_2SO_4 concentration, pH, temperature, and initial sugar concentration on ethanol production in molasses medium.
社団法人日本生物工学会の論文
1987-05-25

凝集性酵母の育種と育種株の発酵特性 : 凝集性酵母によるエタノール生産(第2報)

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