Pseudomonas Putidaによるヒドロキシルアミンの酸化

概要

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Hydroxylamine-oxidizing activity in P. pulida was demonstrated. When fully grown cells of the organism was exposed to 1-3 μg/ml hydroxylamine for an hour, hydroxylamine-oxidizing activity was increased. By rupture of the cells, the hydroxylamine-oxidizing activity in the cell-free extract was decreased to 1/15 that of the viable cells. This activity, however, was doubled in the 0-20% ammonium sulfate fraction of the cell-free extract. On the other hand, reductive activity of nitrite was found in the 50% ammonium sulfate fraction. Nitrite reductase may be one of the causes of the decrease of hydroxylamine-oxidizing activity upon rupture of the cells. Protoplast cells of P. putida had higher enzyme activity than its viable cells. By rupture of the protoplast cells by various methods, differences in the enzyme activity values were demonstrated, along with the degree of rupture.
社団法人日本生物工学会の論文
1988-09-25