工業規模装置によるタバコ植物細胞の連続培養

概要

論文の詳細を見る
To develop the technique of industrial biomass production of tobacco cells by continuous cultivation, many experiments were carried out in 200l and 2,000l fermentors, using the strain of Nicotiana tabacum L. cv. Bright Yellow-2. In this study, the cultivation conditions indicated by the above experimental results were applied to a 20 kl fermentor and the validity of the conditions for stabilizing the continuous culture for a long time was confirmed.The residual sugar content in the culture would be an index of the state in the continuous culture. It was important to maintain value at over 5g/l by controlling the aeration, agitation and dilution rates. An increase of the medium concentration was required for improving the productivity. The maximum value obtained was 5.82 g・l^<-1>・day^<-1>, when the sugar content in the fresh medium was 37 g/l.Morphological change of cell aggregates with time in the unstable state ceased in the stable state and characteristic values became almost constant throughout the run.
社団法人日本生物工学会の論文