機能的筋肉移植術におけるMyoDの発現

概要

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We hypothesize that recovery process after free muscle graft activates regeneration of satellite cells in the grafted muscle. The present study was undertaken to prove the hypothesis using MyoD, one of the myogenic regulatory factors (MRFs), which are known as regulators in the process of muscle regeneration after muscle injuries. Models for muscle graft were created using gastrocunemius muscles of Wister rats. They were divided into three groups. The first group was the reinnervation model in which the tibial nerve received neurorraphy. The second group was the denervation model which received the nerve cut only. The third group was the ischemia model. In all groups, one-hour ischemia was given to the muscles. In these three groups, an expression of mRNA of MyoD was examined by RT-PCR. Electromyography confirmed the reinnervation at 4 weeks after the operation in the reinnervation group. The m-RNA expression of MyoD markedly increased at the time of the reinnervation. On the other hand, the amount of the expression in the denervation group was less than half of the first group at 3 weeks after the operation. Marked expression of MyoD mRNA which correlated with the process of reinnervation in the muscle graft strongly suggested that activation of satellite cells were involved in these processes.
山口大学医学会の論文
2003-12-31