稻熱病菌の榮養生理に關する研究
スポンサーリンク
概要
- 論文の詳細を見る
The nutritional physiology of the rice blast fungus, Piricularia oryzae has been studied with special reference to the growth factors. The constitution of the standard medium used was as follows: NaNO_3 2.0g., KH_2PO_4 0.5g., K_2HPO_4 0.5g., MgSO_4 0.5g., CaCl_2 0.1g., Sucrose 20g., Biotin 5μg., Thiamine hydrochloride 500μg., MoO_4Na_2・2H_2O 0.05mg., Na_2B_4O_7・10H_2O 0.1mg., FeCl_3 0.4mg., CuSO_4・5H_3O 0.4mg., MnSO_4・7H_2O 7.5mg., ZnSO_4・7H_2O 7.5mg., and a liter of distilled water. The followings were shown from the results of the study: 1. Thiamine or both of its two components, pyrimidine and thiazole are essential for the growth of the fungus. If one of the components is absent in the culture solution, the fungus can not grow in it. 2. The minimum requirement of biotin was about 0.004 μg., and that of thiamine hydrochloride was about 0.2 μg. in 10 ml of culture solution. 3. Some of sugars and higher alcohols were tested as the carbon source. The results indicated that sugars were preferable to the higher alcohols for the growth of the fungus in general. Sorbose and inosit were not utilized as the carbon source. 4. Ammonium sulfate, sodium glutamate, asparagine and sodium nitrate were tested as the nitrogen source. In the media containing equivalent amount of nitrogen, the order of rate of growth was found to be: Ammonium sulfate > Sodium glutamate > Asparagine > Sodium nitrate. Among the amino acids present in the rice plant, aspartic and glutamic acids were the most suitable, whereas, tyrosine and phenylalanine were inferior to the other amino acids. When small amoun of asparagine or glutamine was added to the culture solution, the growth of the fungus was somewhat promoted. Aspartic and glutamic acids offered the similar but less effect as above. Some of the organic acids such as malic, succinic, citric and tartaric acids, were effective for the promotion of the fungus growth, and especially the ammonium salts of them were stimulative. 5. Amino acids of the mycelial mat developed in culture solution containing inorganic nitrogen source such as nitrate or ammonium salt, were analyzed by the paper chromatography. The following amino acids are detected by the analyses: Aspartic acid, glutamic acid, glycine, alanine, valine, leucines (leucine and or isoleucine), lysine, arginine, histidine, threonine, proline, tyrosine, phenylalanine and methionine. 6. The nutritional requirements of about fifty isolates of this fungus were compared. There were no differences among them, however, as for the growth factor requirements. By the irradiation of the ultraviolet ray to the spore, a mutant appeared which required adenine in addition to biotin and thiamine as growth factors. This mutant is similar to the mother isolate in spare formation and spore size, but the colour of the mycelia on rice straw decoction agar is slightly deeper than that of the mother isolate. This mutant remained pathogenic to rice plant.
- 日本植物病理学会の論文
- 1953-07-31