Pellicularia filamentosa の代謝毒素の分離

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The present paper deals with the isolation and purification of phytotoxic metabolites produced by Pellicularia filalnentosa causing leaf blight of Ladino clover. By using two methods of assay for necrotic effect upon the leaves and inhibitory effect upon the seedling growth of Ladino clover, it was found that the acidic fraction of the filtrate from Richards' media containing 0.5% peptone, on which the fungus had been grown for about 40days at 28℃, was highly toxic to the host plant. Eight acidic substances (Substance I〜VIII) have been detected so far in the acidic fraction by paper chromatography as shown in Fig. 2, and each of these substnces was further purifled. The results of tests for toxicity showed that Substance I, III, VI and V had a marked toxicity to Ladino clover, as shown in Fig. 3 and Table 1. Judging from some physical and chemical properties, Substance I (m. p. 74℃), IV(m. p. 129℃), V(m. p. 148℃), VI(m. p. 185℃), VII and VIII were identified as phenylacetic acid, m-hydroxy-phenylacetic acid, p-hydroxy-phenylacetic acid, succinic acid, lactic acid and oxalic acid, respectively. Substances II (m. p. 176℃) and III (m. p. 142℃) remain yet to be identified.
日本植物病理学会の論文
1963-09-25