イネ白葉枯病原細菌の培地に関する研究

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概要

• 論文の詳細を見る

• Although Xanthomonas oryzae is usually cultured on ring rot medium, this medium is not suitable for counting the viable number of this bacterium because the colony formation rate varies markedly on this medium. From the results of the study on the variation of colony formation rate, it was found to be influenced by composition, concentration, and pH of the medium, incubation temperature, etc. It was also noticed that variety and place of production of potatoes used was the most important factor. Therefore a synthetic medium that provides a high degree of stability and reappearance of colony formation rate was required. This is accomplished by modification of the synthetic medium, originaly formulated by Mukoo and Watanabe, as follows: Na-glutamate 2g, MgCl 2・6H 20 1g, K 2HP0 4 0.1g,Fe as EDTA salt 1mg, sucrose 5g, distilled water 1 liter; pH was adjusted to 7.O. Bacterial colonies developed well on this modified synthetic agar medium and the colony formation rate was ca 80% when 50〜200 cells were inoculated. A few precautions are necessary in preparation and use of this medium. The Fe-EDTA solu-tion must be renewed once a week in summer and once a month in winter. When viability counts are estimated on this medium, better results are obtained by addition of l% of peptone as the colonies on the synthetic medium with peptone appear faster than those on the synthetic medium alone. This modified synthetic medium is suitable for estimating viability counts but is not suitable for stock culture.

• 日本植物病理学会の論文
• 1962-09-25

著者

• 諏訪 隆之

  北海道立中央農業試験場

• 諏訪 隆之

  農林省農業技術研究所

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• イネ白葉枯病原細菌の培地に関する研究
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