日本梨の黒斑病菌(Alternaria Kikuchiana TANAKA)に對する抵抗性に關する研究 : (第3報)黒斑菌が生成する酸化酵素に就て
スポンサーリンク
概要
- 論文の詳細を見る
1. By means of cultural method the BAVENDAMM'S reaction of Alternaria Kikuchiana, a causal fungus of black spot disease of Japanese pear, is positive on its culture media. The writers have also found the laccase (polyphenol-oxidase) reaction in the fungus-free filtrates of potatodecoction culture, showing blue color with guaiacum tincture. However, we did not find tyrosinase in them. 2. The optimum pH value of polyphenol-oxidase produced by the present fungus lies approximately at 8.0〜8.3, using PARITZSCH'S borate buffer. When potatodecoction media containing 2 % sucrose was used, the maximum value of Oxidase activity was obtained in 12 to 14 days after inoculation, but after about 30 days it was negligible. It is assumed, therefore, that the Oxidase activity is parallel th the growth curve of the mycelium. When different amounts of sugar was added to the media, the activity was most intensive in the culture solution containing 2 % sugar, having no connection with the dry weight of the mycelium. 3. When the culture filtrate was dialysed through collodion semipermeable membrane, we found that the enzyme solution that remained in the bag did not cause the death of leaf tissue and the toxic substances contained in the solution outside the bag, had the ability to cause the death of the tissues showing black appearance. 4. Among the fungi belonging to the genus Alternaria, it was found that some species had positive reaction of Polyphenol-oxidase in their fungus-free filtrates of culture media. Many of them, however, do not attack pear leaves, thus there is no resulting necrosis of cells. 5. From the result of the above stated investigations, it was concluded that Polyphenol-oxidase produced by the present fungus did not cause the death of tissues in leaves and fruits of pear directly. It is presumable, however, that Oxidase has some connection with the process of blackening of the lesions.
- 日本植物病理学会の論文
- 1952-05-31
著者
関連論文
- 日本梨の黒斑病菌(Alternaria Kikuchiana TANAKA)に對する抵抗性に關する研究 : (第3報)黒斑菌が生成する酸化酵素に就て
- (27) 日本梨の黒斑病に對する抵抗性に關する研究(第6報)(昭和28年度秋季関西部会)