(36)Lectins Are Not Involved in Anastomosis Behaviour of Rhizoctonia Species

概要

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Twenty five self anastomosing isolates and five non-self anastomosing isolates from Rhizoctonia solani and 21 self-anastomosing isolates from R. circinata were tested for the presence of surface and tissue lectins. Fifteen days old mycelia cultures on potato dextrose broth at 25℃ were harvested. Crude agglutinins (CA) from homogenized and non-homogenized mycelia, extracted overnight in phosphate buffer (pH, 7.4) at 4℃, were centrifuged, and the supernatants were tested as tissue and surface lectins, respectively. Lectin detection in CA was done using affinity chromatography with protease-treated human and quail erythrocytes. All self-anastomosing isolates from R. circinata tested except one had no traces of lectins. All self-anastomosing and non-self anastomosing isolates from R. solani had lectins detected at high levels. However, out of the 27 R. solani isolates tested, only 11 self-anastomosing and one non self-anastomosing isolates had surface lectins detected at low levels. These results indicate that lectins are not directly involved in the anastomosis process.
日本植物病理学会の論文
2003-02-25