(25)Molecular Epidemiology of Phytoplasma Diseases and DNA Heteroduplex Mobility Assay

概要

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The sensitivity of DNA hetero-duplex mobility assay (HMA) for detecting DNA variability is higher than any other technique currently available, except for exhaustive DNA sequence analysis, and is suitable for studies of phytoplasma epidemiology because of its simplicity and rapidity. In this study HMA of the PCR-amplified 16/23S spacer region was employed to characterize phytoplasmas associated with different ornamental and agricultural crops as well as weeds, shrubs and trees. In Alberta, prevalent phytoplasmas are aster yellows subgroups 16Sr I-A and 16Sr I-B in many plant species. Two subgroups also were identified in each of the phytoplasmas from alfalfa witches'-broom, clover proliferation and potato witches'-broom. The results indicated the widespread occurrence of phytoplasma diseases among annual and perennial crops as well as weeds and trees, and suggested that certain perennial plants and trees as reservors, in addition to vector leafhoppers, could support the continuity of field infections by phytoplasmas.
日本植物病理学会の論文
2003-02-25