Inhibitory Effects of Mulberry Leaf Extract on Postprandial Hyperglycemia in Normal Rats

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We examined the inhibitory effects of aqueous ethanol extract from mulberry leaves (ME) on postprandial hyperglycemia in normal Wistar rats. ME dose-dependently suppressed the postprandial rise of blood glucose in rats, when ME (0.02-0.5 g/kg) was given 0.5 h before the administration of carbohydrates such as sucrose, maltose and starch. The ME dose showing 50% inhibition of the increment of blood glucose (ED_<50>) was 0.11 g/kg for sucrose, 0.44 g/kg for maltose, and 0.38 g/kg for starch. ME and its basic fraction (MB) containing 1-deoxynojirimycin were assayed for their inhibitory effects (IC_<50>) on disaccharidase derived from the small intestine of rats. The IC_<50> value of ME was 3.2 μg/mL for sucrase, 10 μg/mL for isomaltase, and 51 μg/mL for maltase. The IC_<50> value of MB was 0.36 μg/mL for sucrase, 1.1 μg/mL for isomaltase, and 6.2 μg/mL for maltase. The IC_<50> value of 1-deoxynojirimycin as the principle component in ME was 0.015 μg/mL for sucrase and 0.21 μg/mL for maltase, and this value was comparable to the IC_<50> of voglibose. The inhibitory activity of ME in α-amylase was weak. These results suggest that ME strongly suppresses postprandial hyperglycemia after carbohydrate loading by inhibiting the activity of disaccharidases in the small intestine of rats.
日本ビタミン学会の論文