Formation of Embryoid Bodies by Mouse Embryonic Stem Cells on Plastic Surfaces(CELL AND TISSUE ENGINEERING, <Special Issue>Advances in Biomedical Science and Engineering)

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概要

• 論文の詳細を見る

• Mouse embryonic stem (ES) cells were cultured on artificial polymeric biomembranes with a phospholipid polymer (phosphatidylcholine, PC) surface. ES cells aggregated to form an embryoid body (EB) on the PC surface immediately after seeding. Single EBs formed on the PC surface after 3d, and their size was depended on the initial number of cells that were seeded. In contrast, many small EBs with a nonuniform shape formed on a conventional hydrophobic nontreated polystyrene surface. RT-PCR assays of the EBs indicated that cell-cell interactions were enhanced in EBs that formed on the PC surface compared with EBs that formed on the polystyrene surface. The transcription factor Pax6, which is a marker of the differentiation of ES cells to neurons, was not expressed in EBs that formed on the PC surface; however, EBs that formed on the polystyrene surface did express Pax6, indicating that they were undergoing differentiation into neurons. When stimulated with retinoic acid (an inducer of differentiation into neurons), EBs on the PC surface expressed Pax6. We also observed that the adhesion of ES cells to the PC surface was reduced. Thus, the formation of large EBs on the PC surface was due to enhanced cell-cell interaction and inhibition of nonspecific differentiation to neurons.

• 社団法人日本生物工学会の論文
• 2005-07-25

著者

• Konno Tomohiro

  Regenerative Medical Bioreactor Project Kanagawa Academy Of Science And Technology

• Konno Tomohiro

  Regenerative Medical Bioreactor Project Kanagawa Academy Of Science And Technology (kast)

• Ito Yoshihiro

  神奈川科学技術アカデミー 再生医療バイオリアクター

• Kurita Kimio

  Department Of Materials And Applied Chemistry College Of Science And Technology Nihon University

• Ito Yoshihiro

  Regenerative Medical Bioreactor Project, Kanagawa Academy of Science and Technology

• Akita Kunihiko

  Department of Materials and Applied Chemistry, College of Science and Technology, Nihon University

• Konno Tomohiro

  Regenerative Medical Bioreactor Project Kanagawa Acad. Of Sci. And Technol.

• Akita Kunihiko

  Department Of Materials And Applied Chemistry College Of Science And Technology Nihon University

• Ito Yoshihiro

  Regenerative Medical Bioreactor Project Kanagawa Academy Of Science And Technology:nano Medical Engi

関連論文

• Culture of Mouse Embryonic Stem Cells on Photoimmobilized Polymers(CELL AND TISSUE ENGINEERING)
• Nano-technological Control of Biomaterial-Hard Tissue Interfaces(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
• Formation of Embryoid Bodies by Mouse Embryonic Stem Cells on Plastic Surfaces(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
• Ex Vivo Expansion of Human Cord Blood Hematopoietic Progenitor Cells Using Glutaraldehyde-Fixed Human Bone Marrow Stromal Cells(CELL AND TISSUE ENGINEERING)
• Chemically Fixed Nurse Cells for Culturing Murine or Primate Embryonic Stem Cells(CELL AND TISSUE ENGINEERING)
• 14.Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells(12th Annual Meeting of the Society of Hard Tissue Biology)
• A Photo-immobilized Allergen Microarray for Screening of Allergen-specific IgE

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