Culture Systems for Pluripotent Stem Cells(<Special Issue>Advances in Biomedical Science and Engineering)
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概要
- 論文の詳細を見る
Pluripotent stem cells have the capacity to self renew and to differentiate to cells of the three somatic germ layers that comprise an organism. Embryonic stem cells are the most studied pluripotent stem cells. Pluripotent stem cells have also been derived from adult tissues. Both embryonic and adult stem cells represent valuable sources of cells for applications in cell therapy, drug screening and tissue engineering. While expanding stem cells in culture, it is critical to maintain their self-renewal and differentiation capacity. In generating particular cell types for specific applications, it is important to direct their differentiation to the desired lineage. Challenges in expansion of undifferentiated stem cells for clinical applications include the removal of feeder layers and non-defined components in the culture medium. Our limited basic knowledge on the requirements for maintaining pluripotency of adult pluripotent stem cells and the lack of appropriate markers associated with pluripotency hinders the progress toward their wide spread application. In vitro differentiation of stem cells usually produces a mixed population of different cell lineages with the desired cell type present only at a small proportion. Use of growth factors that promote differentiation, expansion or survival of specific cell types is key in controlling the differentiation towards specific cell lineages. A variety of bioreactors for cell cultivation exist and can be readily adapted for stem cell cultivation and differentiation. They provide a well-controlled environment for studying the process of stem cell propagation and differentiation. Their wide use will facilitate the development of processes for stem cell application.
- 社団法人日本生物工学会の論文
- 2005-07-25
著者
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Hu Wei
Department of Epidemiology, Soochow University School of Radiation Medicine and Public Health
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Ulloa Montoya
Department Of Chemical Engineering And Materials Science University Of Minnesota
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Ulloa-montoya Fernando
Department Of Chemical Engineering And Materials Science University Of Minnesota
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Hu Wei-shou
Dep. Of Chemical Engineering And Materials Sci. Univ. Of Minnesota
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Hu Wei
Department Of Epidemiology Soochow University School Of Radiation Medicine And Public Health
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Verfaillie Catherine
Stem Cell Institute and Division of Hematology, Department of Medicine, University of Minnesota
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Hu Wei-shou
Department Of Chemical Engineering And Material Science University Of Minnesota
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Verfaillie Catherine
Stem Cell Institute And Division Of Hematology Department Of Medicine University Of Minnesota
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